Millipore Sigma Vibrant Logo

14-550 MAP Kinase 2/Erk2 Protein, active, Human, 10 µg

Active, N-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2. Activated with MEK1 (Cat. # 14-429). For use in Kinase Assays.

MAP Kinase 2/Erk2 Protein, active, Human, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View This Product on Sigma-Aldrich
14-550
10 µg  Also available in 125μg size, please inquire for pricing and availability.
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue Number14-550
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase 2/Erk2 Protein, active, Human, 10 µg
      OverviewN-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2, Activated with MEK1 (14-429).
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2. Activated with MEK1 (Cat. # 14-429). For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      Protein TargetMAPK2
      Purification MethodGlutathione agarose; Ni2+/NTA agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.8kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceUsed to phosphorylate MBP (13-104)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 125μg size, please inquire for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-550 04053252332470

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-550M MAP Kinase 2/Erk2 Protein, active, Human, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
      Learn More >>

      Erk (MAP) Kinase Antibodies

      Millipore’s Erk Antibodies demonstrate specificity against Erk kinases, p44 and p42 MAP kinases. See below for related products, including proteins & kits based on the expertise of Upstate & Chemicon.
      Learn More >>

      MAPK Antibodies, Proteins, Inhibitors, & Assays

      Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
      Learn More >>

      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases