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17-294

Rho Activation Assay Kit

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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Quality Segment

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable, affinity binding assay: suitable

NCBI accession no.

NM_001664.2

UniProt accession no.

P08134, P61586, P62745

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

dry ice

Gene Information

human ... RHOA(387)

General description

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

Application

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Packaging

Kit capacity: 30 assays

Analysis Note

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

Other Notes

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

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