Quality Segment
form
emulsion (aqueous)
technique(s)
cell culture | hybridoma: suitable, microbiological culture: suitable
density
0.97 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.
Application
Antifoam A concentrate has been used:
- in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
- to prevent foam formation during the aerobic treatment of sludge.
- to study the impact of surface tension on bioaerosol generation during bubbling.
Features and Benefits
- Molecular biology grade and tested for use in bacterial fermentation.
- Extremely effective foam suppressor for aqueous and non-aqueous systems.
- Made of 100% active silicone polymer, free of emulsifiers.
- Typically, effective at 1-100 ppm.
- Product will be stable in the pH range of 5 to 9.
- Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.
Preparation Note
Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.
Other Notes
Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .
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signalword
Danger
hcodes
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
>213.8 °F - closed cup
flash_point_c
> 101 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - PBT - vPvB