biological source
synthetic
Quality Segment
form
suspension
contains
emulsifier
technique(s)
cell culture | hybridoma: suitable, microbiological culture: suitable
viscosity
400 cP
mp
0 °C (32 °F)
density
1.01 g/mL
application(s)
(Supplement in culture media)
General description
Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.
Application
Antifoam C emulsion has been used:
- as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
- as a component of feed medium,
- as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
- as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions
Features and Benefits
- Minimizes compatibility issues with biological systems
- Highly effective defoamer at low concentrations
- Highly effective and versatile silicone defoamer
Other Notes
A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter