Quality Segment
form
emulsion (aqueous)
concentration
10% (active silicon)
technique(s)
cell culture | hybridoma: suitable
solubility
H2O: soluble (effective hot or cold.)
density
1.0 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Application
Antifoam SE-15 has been used:
- in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
- to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
- as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
- as a component of autoinduction expression culture.
- as a component of cyclic adenosine monophosphate (cAMP) detection buffer.
Features and Benefits
- Controls foaming in microbial culture flasks or fermentations
- Non-toxic to the culture medium
- Can be pumped to fermenter on an as-needed basis
Preparation Note
Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.
suspensions should be used immediately.
Other Notes
Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT RE 2 Inhalation
target_organs
Lungs
저장 등급
12 - Non Combustible Liquids