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MDQ1

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Synonym(s):

DNA Methylation Quantification, DNA methylation shift

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About This Item

NACRES:
NA.54
UNSPSC Code:
41116158
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General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • M8570
    Methylated Control DNA, 50 ng/μL
    SDS

pictograms

CorrosionExclamation mark
GHS05,GHS07

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Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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Protocols

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Articles

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.

Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
Resistance training and redox homeostasis: Correlation with age-associated genomic changes
Dimauro I, et al.
Redox Biology, 10, 34-44 (2016)
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