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P7367

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE)

Synonym(s):

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

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About This Item

CAS Number:
83534-39-8
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
3.5.1.52 (BRENDA, IUBMB)
MDL number:
MFCD18839214
Assay:
≥95% (SDS-PAGE)
Biological source:
bacterial (Elizabethkingia meningoseptica)
Concentration:
≥300 units/mL, ≥50 units/mL
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biological source

bacterial (Elizabethkingia meningoseptica)

Quality Segment

200

conjugate

(N-linked)

product line

BioReagent

assay

≥95% (SDS-PAGE)

form

powder

shelf life

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml), ≥1 yr at 2‑8 °C, Solution is stable for at least 3 freeze-thaw cycles

mol wt

~36 kDa

concentration

≥300 units/mL, ≥50 units/mL

optimum pH

~8.6

shipped in

wet ice

storage temp.

2-8°C

General description

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Application

PNGase F from Elizabethkingia meningoseptica has been used:
  • for de-N-glycosylation of Zika20virus E protein
  • to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
  • to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Storage Class

11 - Combustible Solids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P7367-300UN-PW: + P7367-50UN: + P7367-BULK: + P7367-300UN: + P7367-1VL: + P7367-BULK-N: + P7367PROC: + P7367-10UN-PW: + P7367-50UN-KC: + P7367-PH: + P7367-10UN: + P7367-VAR: + P7367-50UN-PW: + P7367-25UN:

jan

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