Skip to Content
Merck

S4438

SYBR® Green JumpStart™ Taq ReadyMix™

for quantitative PCR, MgCI2 in buffer

Synonym(s):

Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
41106300
NACRES:
NA.55
MDL number:
MFCD03458666
Concentration:
1.25 units/reaction (50 μL reaction volume)

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 500 reactions

feature

dNTPs included, hotstart

concentration

1.25 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

input

purified DNA

suitability

suitable for (quantitative PCR)

application(s)

agriculture

compatibility

ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT Fast, ABI 7900 HT, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7 , Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2 , Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ 5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex , Eppendorf® Mastercycler ep realplex2 S, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480 , Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000, for use with ABI 5700

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

form

liquid

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

SYBR® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling.
SYBR® Green JumpStart™ Taq ReadyMix™ combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

Application

SYBR® Green JumpStart™ Taq ReadyMix™ has been used:

  • in quantitative PCR (qPCR) to amplify a single area of conservation from all variants of myelin basic protein (mbp), SRY-Box 10 (sox10), and β-actin genes
  • in quantitative real-time PCR to detect the accumulation of PCR product at every cycle of amplification
  • in routine qPCR amplifications of DNA template and cDNA template
  • for amplification of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR)

Biochem/physiol Actions

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Features and Benefits

  • Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required
  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments
  • This master mix allows consistency from one reaction to the next
  • Designed to minimize contamination
  • Reduced primer dimers
  • Reduced set-up time as compared to manual or wax HotStart® methods
  • Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf SE
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

Please refer to KIT Component information

prtr

Please refer to KIT Component information

fsl

Please refer to KIT Component information

ishl_indicated

Please refer to KIT Component information

ishl_notified

Please refer to KIT Component information

cart

キットコンポーネントの情報を参照してください

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000523966
0000523966
0000488450
0000488669
0000488471

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Karim Sorefan et al.
Nature, 459(7246), 583-586 (2009-05-30)
Local hormone maxima are essential for the development of multicellular structures and organs. For example, steroid hormones accumulate in specific cell types of the animal fetus to induce sexual differentiation and concentration peaks of the plant hormone auxin direct organ
Mathew S Box et al.
Plant methods, 7, 7-7 (2011-03-15)
Many experiments in modern plant molecular biology require the processing of large numbers of samples for a variety of applications from mutant screens to the analysis of natural variants. A severe bottleneck to many such analyses is the acquisition of
Temporal dynamics of myelination in the zebrafish spinal cord
Buckley C E, et al.
Glia, 58(7), 802-812 (2010)
Blair R G Gordon et al.
Journal of bacteriology, 190(21), 7052-7059 (2008-09-09)
Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Our previous in vitro biochemical studies showed that Lsr2 is a DNA-bridging protein, a property shared by H-NS-like proteins in gram-negative bacteria. Here we present in vivo evidence
Bikem Soygur et al.
Human reproduction (Oxford, England), 31(7), 1455-1461 (2016-05-14)
As Syncytin 1 (human endogenous retrovirus (HERV-W)) is crucial for human embryo placentation is it expressed during preimplantation embryo development? Syncytin 1 was expressed mainly in trophoblast cells of the blastocyst particularly in cells underlying the inner cell mass (ICM).
View All Related Papers

Articles

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

How qPCR Works

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

See All

Protocols

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

Related Content

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

RT-qPCR – 定量的逆転写PCR

RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service