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T3038

Trizma® hydrochloride solution

1 M, BioReagent, Molecular Biology

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
PubChem Substance ID:
24900102
UNSPSC Code:
12161700
MDL number:
MFCD00012590
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grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

8.0

application(s)

agriculture

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T3038-PH: + T3038PROC: + T3038-BULK: + T3038-1L:4548173212654 + T3038-VAR: + T3038-100ML-KC: + T3038-40ML:4548173212661 + T3038-100ML:4548173212647 + T3038-EW: + T2694-100ML-PW: + T3038-1L-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000537455
0000537455
0000257167
0000364846
0000455980

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