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06-570-AF647

Anti-phospho Histone H3 (Ser10), Alexa Fluor™ 647 Conjugate Antibody

0.5 mg/mL, from rabbit

Synonym(s):

H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 647
Clone:
polyclonal
Application:
ICC
Citations:
1
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biological source

rabbit

Quality Segment

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

concentration

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_003484.1

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 06-570.
~17 kDa observed (Refer to Cat. No. 06-570)

Immunogen

Linear peptide corresponding to Histone H3 phosphorylated at Ser10.

Application

Anti-phospho-H3 (Ser10), Alexa Fluor™ 647 Conjugate is a purified rabbit polyclonal antibody to detect Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 647 and validated for use in IC. The unconjugated version of this antibody is published and proven in ICC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Physical form

Protein A purified
Purified rabbit conjugated antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.

Alexa Fluor™ is a registered trademark of Life Technologies.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

06-570-AF647:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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