Anti-GFP Antibody, clone 3F8.2

28 kDa

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Epitope: Full Length

Full length GFP protein.

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Reacts with most common vertebrate proteins containing the GFP tag.

The antibody reacts with the 28 kDa GFP protein.

Format: Purified

Protein G Purified

Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Western Blot: GFP recombinant protein

Routinely evaluated by Western Blot on GFP recombinant protein.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.