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MABD90

Anti-HLA-DRB1 Antibody, clone DJ9

clone DJ9, from mouse

Synonym(s):

Major histocompatibility complex, class II, DRB1, DRB1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
DJ9, monoclonal
Application:
FACS, ICC, IHC, WB
Citations:
1
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DJ9, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

NCBI accession no.

NP_002112

UniProt accession no.

P01911

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HLA-DRB1(3123)

General description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells and presents them on the cell surface for recognition by the CD4 T-cells. Class II molecules are expressed in antigen presenting cells B lymphocytes, dendritic cells, macrophages. HLA-Drb1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. HLA-DRB1, clone DJ9, has been shown to be a relevant marker of bone marrow derived mesenchymal stem cells and their osteogenic derivatives (Andersen, D., et al., 2011).

The previously assigned protein identifier Q5Y7A7 has been merged into P01911. Full details can be found on the UniProt database.
~30 kDa observed

Immunogen

Whole hMSC-TERT Cells

Application

Detect HLA-DRB1 using this Anti-HLA-DRB1 Antibody, clone DJ9 validated for use in Western Blotting, ICC, IHC, Flow Cytometry.
Research Category
Inflammation & Immunology

Stem Cell Research
Research Sub Category
Immunological Signaling

Mesenchymal Stem Cells
This antibody has been shown to work in Immunocytochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Immunohistochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Flow Cytometry (Andersen, D., et al. (2011)).

Biochem/physiol Actions

This antibody recognizes human HLA-DRB1.

Physical form

Format: Purified

Analysis Note

Control
Daudi cell lysates
Evaluated by Western Blot in Daudi cell lysates. Western Blot Analysis: A 1:100,000 dilution of this antibody detected HLA-DRB1 in Duadi cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABD90:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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