Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7

Cryptococcus neoformans is an encapsulated yeast-like fungus that is reported to causes systemic infections, including fatal meningoencephalitis. Glucuronoxylomannan (GXM) is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro. The basic structural unit of GXM is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection, and high levels of GXM have been found in serum of patients with cryptococcosis. Clone 18B7 displays significant activity against GXM and is reported act by possibly hydrolyzing the oligosaccharide substrate. This antibody acts as a potent opsonin for Cryptococcus neoforman by increasing its phagocytosis. This antibody may also reduce GXM levels by interfering with the release of polysaccharide from the capsule. Administration of this antibody to mice is shown to rapidly clear serum Cryptococcal antigen. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Martinez, LR., et al. (2004). Infect. Immun. 72(6); 3674 3679; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Devi, SJN et al. (1991). Infect. Immun. 59(10); 3700-3707).

Cryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.

Anti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),

Immunohistochemistry Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunohistochemistry applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46).

Immunodepletion Analysis: A representative lot immunodepleted Glucuronoxylomannan (GXM) in Immunodepletion applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

Immunofluorescence Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunofluorescence applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues.

ELISA Analysis: A representative lot detected Glucuronoxylomannan (GXM) in ELISA applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

Research Category
Inflammation & Immunology

Clone 18B7 is a mouse monoclonal antibody that binds to Glucuronoxylomannan (GXM) from Cryptococcus neoformans.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.