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MABN1844

Anti-Aldolase C Antibody, C-Term, clone 1A1

clone 1A1, from mouse

Synonym(s):

Fructose-bisphosphate aldolase C, Brain-type aldolase, Aldolase C, C-Term

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1A1, monoclonal
Application:
ICC, WB
Citations:
-
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A1, monoclonal

species reactivity

human, rat

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_005156

UniProt accession no.

P09972

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ALDOC(230)

General description

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.
~39 kDa observed

Immunogen

Epitope: Near C-terminus; 23 amino acids of aldolase C protein
Recombinant protein corresponding to human Aldolase C near the C-terminus.

Application

This Anti-Aldolase C Antibody, C-Term, clone 1A1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Aldolase C in 10 µg of human brain tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

Physical form

Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

Analysis Note

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Aldolase C in 10 µg of rat brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABN1844:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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