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OP146

Anti-MDM2 (Ab-6) Mouse mAb (5B10C)

Name: Anti-MDM2 (Ab-6) Mouse mAb (5B10C)
Brand: MM

liquid, clone 5B10C, Calbiochem^®

Synonym(s):

Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2

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About This Item

UNSPSC Code:

12352203

Clone:

5B10C, monoclonal

Species reactivity:

human

Application:

—

Citations:

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Properties

Quality Segment

100

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

5B10C, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MDM2(4193)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MMDM2 protein.

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.

Immunogen

Human

recombinant human MDM2

Application

Immunoblotting (2 g/ml)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction, see application references)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A549 cells or breast carcinoma tissue

Other Notes

Antibody should be titrated for optimal results in individual systems.

Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO J.10, 1565.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

OP146-20SUG: + OP146-MG: + OP146-ML: + OP146-100UG:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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