SCC300
3dGRO® Human iPSC Derived Colon Organoids
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About This Item
form
liquid
technique(s)
cell culture | stem cell: suitable
application(s)
cell analysis
Analysis Note
- Each vial contains ≥1500 organoids.
- Mycoplasma: Negative
- Genotyped by STR analysis: Pass
- Human species verified
- Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
- Cell viability test: Pass
Application
Research Category:
- Cell Culture
- Stem Cell Research
Disclaimer
General description
3dGRO® Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO® human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO® Human Colon Organoid Expansion Medium (Cat. No. SCM304).
Preparation Note
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
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Articles
ヒト上皮性結腸オルガノイドは、Caco-2細胞の薬物透過性アッセイの代わりに、薬物スクリーニングおよび化合物毒性試験に使用できます。
オルガノイド培養に関するFAQ3D細胞培養プロトコルに従ったオルガノイドの培養・分析方法をご覧ください。ヒントとコツ。
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
Protocols
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
優れた特性を有する凍結保存状態のヒトiPS細胞由来腸オルガノイドと、iPS細胞から上皮性腸オルガノイドへ分化させるための段階的なオルガノイド培養のプロトコル。
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Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Monitor barrier formation using colon PDOs, iPSC-derived colon organoids, Millicell® cell culture inserts, and the Millicell® ERS. 3.0.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC300 | 04054839989728 |
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