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Merck

AEC101

AEC Staining Kit

liquid

Synonym(s):

ACE substrate kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116134
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form

liquid

Quality Segment

usage

sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.

2-8°C

Application

AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Disclaimer

Stable at least one year at 2-8 °C.


signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

134.6 °F - closed cup

flash_point_c

57 °C - closed cup


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

Please refer to KIT Component information

prtr

Please refer to KIT Component information

fsl

Please refer to KIT Component information

ishl_indicated

Please refer to KIT Component information

ishl_notified

Please refer to KIT Component information

cart

キットコンポーネントの情報を参照してください

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Protocols

パラフィン包埋組織切片中の特定の抗原を染色および可視化する免疫組織染色(IHC)の全プロトコルをご紹介します。

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.


Kaiyuan Ni et al.
Chem, 5(7), 1892-1913 (2019-08-07)
Checkpoint blockade immunotherapy (CBI) elicits durable therapeutic responses by blocking T cell inhibitory pathways of tumors with pre-infiltrated T cells and/or high mutational burden to activate antitumor immunity but is ineffective against poorly immunogenic tumors. Immunogenic radiotherapy, photodynamic therapy (PDT)
Leslie R Morse et al.
Translational stroke research, 2(4), 643-650 (2012-03-01)
Spinal cord injury is associated with rapid bone loss and arrested long bone growth due to mechanisms that are poorly understood. In this study, we sought to determine the effects of severe T10 contusion spinal cord injury on the sublesional
Identification of early secretory antigen target-6 epitopes for the immunodiagnosis of active tuberculosis
Vincenti D, et al.
Molecular Medicine, 9(3), 105-105 (2003)