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D0426

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

Synonym(s):

3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name

SIGMAFAST™ DAB with Metal Enhancer, tablet

form

tablet

Quality Segment

200

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

General description

SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Application

The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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キットコンポーネントの情報を参照してください

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Certificates of Analysis (COA)

Lot/Batch Number
SLCD4801
SLBP7387V
SLBJ7196V
SLBF4765V
061M8207V

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Protocols

免疫組織染色のプロトコル

パラフィン包埋組織切片中の特定の抗原を染色および可視化する免疫組織染色（IHC）の全プロトコルをご紹介します。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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