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P3563

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Synonym(s):

1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST

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About This Item

UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25
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Product Name

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,

form

powder

Quality Segment

200

color

white

solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

cell analysis
diagnostic assay manufacturing

General description

Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Application

Phosphate buffered saline has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
  • cell isolation

Packaging

Foil pouches

Preparation Note

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P3563-1PAK: + P3563-10PAK-KC: + P3563-5X10PAK: + P3563-1EA-KC: + P3563-VAR: + P3563-BULK: + P3563-1EA: + P3563-10PAK:

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