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P9172

Pyronin Y

for NA electrophoresis

Synonym(s):

Pyronin J, Pyronin Y, Pyronin G

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About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
57654569
MDL number:
MFCD00011725
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005
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grade

Molecular Biology

Quality Segment

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P9172-BULK: + P9172-VAR: + P9172-1G: + P9172-5G:

jan

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