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Merck

Y1501

Yeast Synthetic Drop-out Medium Supplements

without uracil

Synonym(s):

Synthetic drop-out medium for yeast

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About This Item

UNSPSC Code:
12352200
NACRES:
ND.02
MDL number:
MFCD01095679

Key Documents

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form

powder

application(s)

food and beverages

Quality Level

200

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Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Preparation Note

1.92 g will supplement 1 liter of medium.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids are present at a concentration of 76 mg/L except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Y1501-20G: + Y1501-1G-PW: + Y1501-VAR: + Y1501-20G-PW: + Y1501-BULK: + Y1501-1G-KC: + Y1501-1G:

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Certificates of Analysis (COA)

Lot/Batch Number
0000508911
0000508911
0000435099
0000435099
0000435093

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Karissa L Cross et al.
mBio, 9(2) (2018-03-15)
The human oral microbiota encompasses representatives of many bacterial lineages that have not yet been cultured. Here we describe the isolation and characterization of previously uncultured Desulfobulbus oralis, the first human-associated representative of its genus. As mammalian-associated microbes rarely have
Vinicius Henrique De Oliveira et al.
Ecotoxicology and environmental safety, 202, 110917-110917 (2020-08-18)
Cadmium (Cd) is an extremely toxic environmental pollutant with high mobility in soils, which can contaminate groundwater, increasing its risk of entering the food chain. Yeast biosorption can be a low-cost and effective method for removing Cd from contaminated aqueous
Xiyan Li et al.
PloS one, 10(12), e0146152-e0146152 (2015-12-31)
Metformin, a leading drug used to treat diabetic patients, is reported to benefit bone homeostasis under hyperglycemia in animal models. However, both the molecular targets and the biological pathways affected by metformin in bone are not well identified or characterized.
Dhammika H M L P Navarathna et al.
PloS one, 11(10), e0164449-e0164449 (2016-10-12)
Formation of chlamydospores by Candida albicans was an established medical diagnostic test to confirm candidiasis before the molecular era. However, the functional role and pathological relevance of this in vitro morphological transition to pathogenesis in vivo remain unclear. We compared
J Abraham Avelar-Rivas et al.
Frontiers in genetics, 11, 468-468 (2020-06-02)
The chronological lifespan of budding yeast is a model of aging and age-related diseases. This paradigm has recently allowed genome-wide screening of genetic factors underlying post-mitotic viability in a simple unicellular system, which underscores its potential to provide a comprehensive
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Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

タンパク質発現系

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Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protocols

酵母形質転換のプロトコル

酵母は成長が速く、細胞が分散しているため、真核生物の研究に適したモデル生物と考えられています。

Yeast Drop Out

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Yeast Growth Methods

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Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Related Content

Data Sheet - Y1501

Instructions

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