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MABE1018 Anti-BBF2H7/CREB3L2 Antibody, clone 28G9

MABE1018
100 μg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H WB, IP, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE1018
      DescriptionAnti-BBF2H7/CREB3L2 Antibody, clone 28G9
      Alternate Names
      • Cyclic AMP-responsive element-binding protein 3-like protein 2
      • BBF2H7
      • cAMP-responsive element-binding protein 3-like protein 2/CREB3L2
      Background InformationCyclic AMP-responsive element-binding protein 3-like protein 2 (UniProt Q8BH52; also known as cAMP-responsive element-binding protein 3-like protein 2) is encoded by the Creb3l2 (also known as Bbf2h7, C530025K05Rik, SCIRR69) gene (Gene ID 208647) in murine species. Creb3l2/Bbf2h7 belongs to the old astrocyte specifically induced substance (OASIS) family of ER-resident transcription factors that function as ER stress transducers. OASIS members are ER transmembrane proteins that possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain. Upon initial activation through regulated intramembrane proteolysis (RIP), OASIS family members are transported from the ER to the Golgi apparatus. Sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P) releases the N-terminal fragment, which then translocates to the nucleus and activates the transcription of target genes. Under normal conditions, cellular BBF2H7 and OASIS levels are low due to degradation via the ubiquitin-proteasome pathway as a result of constant E3 ubiquitin ligase HRD1- (HMG-CoA reductase degradation 1-) mediated ubiquitination. ER stress is shown to disrupt their interaction with HRD1, resulting in enhanced stability of BBF2H7 and OASIS and an upregulation of their target genes.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of BBF2H7/CREB3L2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.25 µg/mL from a representative lot detected BBF2H7/CREB3L2 in 10 µg of mouse MC3T3-E1 preosteoblasts.
      Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
      Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
      Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse BBF2H7/CREB3L2.
      EpitopeN-terminus
      Clone28G9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Creb3l2
      • Bbf2h7
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~57-60 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat C6 glioma cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 試験成績書(CoA)

      タイトルロット番号
      Anti-BBF2H7, clone 28G9 - 32678853267885
      Anti-BBF2H7, clone 28G9 -Q2586393Q2586393

      参考資料

      参考資料の概要Pub Med ID
      Activation of OASIS family, ER stress transducers, is dependent on its stabilization.
      Kondo, S; Hino, SI; Saito, A; Kanemoto, S; Kawasaki, N; Asada, R; Izumi, S; Iwamoto, H; Oki, M; Miyagi, H; Kaneko, M; Nomura, Y; Urano, F; Imaizumi, K
      Cell death and differentiation  19  1939-49  2012

      概要を表示する
      22705851 22705851

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