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CA1023 Anti-Hsp90α Mouse mAb (EMD-17D7)

CA1023
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概要

Replacement Information

主要スペック表

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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CA1023-50UGCN
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      樹脂アンプル 50 μg
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      Description
      OverviewRecognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
      Catalogue NumberCA1023
      Brand Family Calbiochem®
      SynonymsAnti-Heat Shock Protein 90α
      Application Data
      Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.

      Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
      References
      ReferencesScheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
      Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
      Nemoto, T., et al. 1998. Biochem. J. 330, 989.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlH460 and HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (see comments)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (5 µg/0.7 mg protein)
      Application CommentsDetects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenHsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901)
      ImmunogenHuman
      CloneEMD-17D7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Hsp90α Mouse mAb (EMD-17D7) (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-Hsp90α Mouse mAb (EMD-17D7) 試験成績書(CoA)

      タイトルロット番号
      CA1023

      参考資料

      参考資料の概要
      Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
      Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
      Nemoto, T., et al. 1998. Biochem. J. 330, 989.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Heat Shock Protein 90α
      ApplicationELISA (see comments)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (5 µg/0.7 mg protein)
      Application Data
      Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.

      Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
      DescriptionProtein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
      BackgroundHsp90α is involved in signal transduction, cell cycle progression, and apoptosis. Hsp90α has been reported to interact with MMP-2 and promote its activation, which may, in turn, play a role in cancer cell invasiveness.
      HostMouse
      Immunogen speciesHuman
      ImmunogenHsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901)
      CloneEMD-17D7
      IsotypeIgG₁
      Specieshuman
      Positive controlH460 and HeLa cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDetects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesScheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
      Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
      Nemoto, T., et al. 1998. Biochem. J. 330, 989.