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MABE363 Anti-Mili/Piwil-2 Antibody, clone 13E-3

MABE363
100 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB, IP, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE363
      DescriptionAnti-Mili/Piwil-2 Antibody, clone 13E-3
      Alternate Names
      • Piwi-like protein 2
      Background InformationAs a member of the piwi gene family, Mili/Piwil-2 plays an important role in various biological processes such as spermatogenesis, RNA silencing, gametogenesis, maintaining germline integrity, meiotic differentiation of spermatocytes, and germ cell differentiation. Mili/Piwil-2 has been implicated in transposon control through its direct interaction with piwi-interacting RNAs (piRNAs). It is localized in the cytoplasm of spermatogonia, early spermatocytes, and testicular germ cells, where it is enriched in chromatoid bodies. It is also expressed in certain types of somatic stem/progenitor cells. Furthermore, it forms an RNA-independent complex with eIF3a and interacts with the eIF4E- and eIF4G-containing m7G cap-binding complex.
      References
      Product Information
      FormatPurified
      Control
      • Mouse testis tissue lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Mili/Piwil-2 antibody is validated for use in wb, IP & ICC for the detection of the Mili/Piwil-2 protein.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: A representative lot detected in Mili/Piwil-2 in mouse testis tissue lysates (Reuter, M., et al. (2009). Nat Struct Mol Biol. 16(6):639-646.).

      Immunoprecipitation Analysis: A representative lot detected Mili/Piwil-2 in HEK293T cell extracts transfected with HA-Mili (Reuter, M., et al. (2009). Nat Struct Mol Biol. 16(6):639-646.).

      Immunofluorescence Analysis: A representative lot detected Mili/Piwil-2 in mouse germ cells expressing heterozygous Tdrd1 and homozygous mutant Tdrd1 (Reuter, M., et al. (2009). Nat Struct Mol Biol. 16(6):639-646.).
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse Mili/Piwil-2.
      Clone13E-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Piwil2
      • Mili
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse testis tissue lyaste.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Mili/Piwil-2 in 10 µg of mouse testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Mili/Piwil-2 Antibody, clone 13E-3 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-Mili/Piwil-2 Antibody, clone 13E-3 試験成績書(CoA)

      タイトルロット番号
      Anti-Mili/Piwil-2, clone 13E-3 - Q2116530Q2116530
      Anti-Mili/Piwil-2, clone 13E-3 - 32082103208210
      Anti-Mili/Piwil-2, clone 13E-3 -25030312503031
      Anti-Mili/Piwil-2, clone 13E-3 -28092882809288
      Anti-Mili/Piwil-2, clone 13E-3 Monoclonal Antibody3117808
      Anti-Mili/Piwil-2, clone 13E-3 Monoclonal Antibody2942472

      参考資料

      参考資料の概要アプリケーションPub Med ID
      The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements.
      De Fazio, Serena, et al.
      Nature, 480: 259-63 (2011)  2011

      概要を表示する
      Immunocytochemistry, Immunoprecipitation22020280 22020280
      Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile.
      Reuter, Michael, et al.
      Nat. Struct. Mol. Biol., 16: 639-46 (2009)  2009

      概要を表示する
      Immunoprecipitation, Western Blotting19465913 19465913

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies