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IF01 Anti-Vimentin/LN6 (Ab-1) Mouse mAb (V-9)

IF01
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概要

Replacement Information

主要スペック表

Species ReactivityHostAntibody Type
Ch, H, Porcine, RMMonoclonal Antibody

価格&在庫状況

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IF01-100UGCN
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      樹脂アンプル 100 μg
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~58 kDa vimentin protein. Does not cross-react with closely related intermediate filament proteins including desmin and GFA.

      Catalogue NumberIF01
      Brand Family Calbiochem®
      References
      ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N., et al. 1983. FEBS 163, 22.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlCEM-CCRF cells
      Positive controlHeLa, IMR90, or WI38 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesCross-Reactivity Osborn, M., et al. 1984. Eur. J. Cell Biol. 34, 137. Immunostaining Caselitz, J., et al. 1983. Virch. Arch. 400, 43.
      Key Applications Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesFrozen sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml, indirect)
      Paraffin sections (1-5 µg/ml)
      Application CommentsDoes not cross-react with closely related intermediate filament proteins including desmin and GFA (see application references). This antibody stains intermediate filaments found in chondrocytes, fibroblasts, and connective tissue cells, as well as those found in fibroblast-derived cell lines (such as human WI38) and in certain classes of human tumors, including malignant melanomas (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified porcine vimentin
      ImmunogenPorcine
      CloneV-9
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Chicken
      • Human
      • Porcine
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Vimentin/LN6 (Ab-1) Mouse mAb (V-9) 試験成績書(CoA)

      タイトルロット番号
      IF01

      参考資料

      参考資料の概要
      Droese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N., et al. 1983. FEBS 163, 22.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-May-2009 JSW
      ApplicationFrozen sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml, indirect)
      Paraffin sections (1-5 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells. Recognizes the ~58 kDa vimentin protein.
      BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (GFA), skeletal, visceral and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). A few well-defined cell types coexpress vimentin along with one other intermediate filament type. Vimentin (Ab-1) stains the vimentin subclass of intermediate filaments in human tissues.
      HostMouse
      Immunogen speciesPorcine
      Immunogenpurified porcine vimentin
      CloneV-9
      IsotypeIgG₁
      Specieschicken, human, not mouse, porcine, rat
      Positive controlHeLa, IMR90, or WI38 cells
      Negative controlCEM-CCRF cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with closely related intermediate filament proteins including desmin and GFA (see application references). This antibody stains intermediate filaments found in chondrocytes, fibroblasts, and connective tissue cells, as well as those found in fibroblast-derived cell lines (such as human WI38) and in certain classes of human tumors, including malignant melanomas (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N., et al. 1983. FEBS 163, 22.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      Application referencesCross-Reactivity Osborn, M., et al. 1984. Eur. J. Cell Biol. 34, 137. Immunostaining Caselitz, J., et al. 1983. Virch. Arch. 400, 43.