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MABE365 Anti-acetyl PARP1 Antibody, clone E4

MABE365
100 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE365
      DescriptionAnti-acetyl PARP1 Antibody, clone E4
      Alternate Names
      • Poly [ADP-ribose] polymerase 1
      • PARP-1
      • ADP-ribosyltransferase diphtheria toxin-like 1
      • ARTD1
      • NAD(+) ADP-ribosyltransferase 1
      • ADPRT 1
      • Poly[ADP-ribose] synthase 1
      Background InformationPoly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.
      References
      Product Information
      FormatPurified
      Control
      • Acetylated PARP1 fragment (373-529) purified protein lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).
      Biological Information
      ImmunogenLinear peptide corresponding to acetylated human PARP1, short fragment.
      CloneE4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PARP1
      • ADPRT
      • PPOL
      • PARP
      • ARTD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-acetyl PARP1 Antibody, clone E4 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-acetyl PARP1 Antibody, clone E4 試験成績書(CoA)

      タイトルロット番号
      Anti-Modified Citrulline, clone C4 -25472132547213
      Anti-acetyl PARP1, clone E4 - Q2165959Q2165959
      Anti-acetyl PARP1, clone E4 -25472332547233

      参考資料

      参考資料の概要Pub Med ID
      Sumoylation of poly(ADP-ribose) polymerase 1 inhibits its acetylation and restrains transcriptional coactivator function.
      Messner, Simon, et al.
      FASEB J., 23: 3978-89 (2009)  2009

      概要を表示する
      19622798 19622798

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies