コンテンツへスキップ
Merck

227036

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

別名:

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free

ログインで組織・契約価格をご覧ください。

サイズを選択してください

この商品について

CAS番号:
9012-63-9
UNSPSC Code:
12352202
NACRES:
NA.51
MDL number:
MFCD00130776
Assay:
≥95% (SDS-PAGE)
Form:
solid
Quality level:
100
Storage condition:
do not freeze

主要文書

すべての文書を表示
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします

製品名

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.

description

RTECS - LF3100000

assay

≥95% (SDS-PAGE)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

solubility

soluble (Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.)

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

関連するカテゴリー

General description

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
Useful for tissue culture applications.

Biochem/physiol Actions

Cell permeable: no
Primary Target
α-subunit of G-proteins
Product does not compete with ATP.
Reversible: no

Packaging

Please refer to vial label for lot-specific concentration.
yes

Physical form

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

Analysis Note

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
single band by disc gel electrophoresis

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

Legal Information

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2

保管分類

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

227036-MG: + 227036-1MG:

jan

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

グローバルトレードアイテム番号

カタログ番号GTIN
227036-1MGCN04055977200560

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）