407707
PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb
liquid, Calbiochem®
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この商品について
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
—
Citations:
9
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
phosphorylation (pTyr1162/pTyr1163)
Quality Level
Gene Information
human ... INSR(3643)
General description
Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr1162/1163.
Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
This PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
Application
ELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.
The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
407707-10T:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tatsuya Iso et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(11), 2549-2557 (2013-08-24)
Fatty acids (FAs) are the major substrate for energy production in the heart. Here, we hypothesize that capillary endothelial fatty acid binding protein 4 (FABP4) and FABP5 play an important role in providing sufficient FAs to the myocardium. Both FABP4/5
Ana S Martins et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(11 Pt 1), 3532-3540 (2006-06-03)
Ewing tumor cell survival and proliferation depends on several autocrine loops. Targeting these loops is a promising therapeutic approach. We recently showed the cytostatic role of imatinib, an inhibitor of the SCF-KIT loop, on Ewing tumor cells, and in this
Natalie J Haywood et al.
Diabetes, 66(2), 287-299 (2017-01-22)
Low circulating levels of insulin-like growth factor binding protein 1 (IGFBP-1) are associated with insulin resistance and predict the development of type 2 diabetes. IGFBP-1 can affect cellular functions independently of IGF binding through an Arg-Gly-Asp (RGD) integrin-binding motif. Whether
Ana Paula Arruda et al.
eLife, 6 (2017-12-16)
Defective Ca2+ handling is a key mechanism underlying hepatic endoplasmic reticulum (ER) dysfunction in obesity. ER Ca2+ level is in part monitored by the store-operated Ca2+ entry (SOCE) system, an adaptive mechanism that senses ER luminal Ca2+ concentrations through the
Ekin Guney et al.
Science signaling, 14(713), eabf2059-eabf2059 (2021-12-15)
Chronic metabolic inflammation is a key feature of obesity, insulin resistance, and diabetes. Here, we showed that altered regulation of the Ca2+ channel inositol trisphosphate receptor (IP3R) was an adipocyte-intrinsic event involved in the emergence and propagation of inflammatory signaling
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 407707-10TCN | 04055977211474 |
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