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69952 pET-31b(+) DNA - Novagen

69952
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概要

Replacement Information

価格&在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
69952-3CN
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      樹脂アンプル 10 μg
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      Description
      OverviewThe pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB098).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69952
      Brand Family Novagen®
      References
      References1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-31b(+) DNA - Novagen 試験成績書(CoA)

      タイトルロット番号
      69952

      参考資料

      参考資料の概要
      1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.

      カタログ

      タイトル
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      引用

      Title
    • Graeme S. Garvey, Susan P. McCormick and Ivan Rayment. 2008. Journal of Biological Chemistry 283, 1660.
    • Kim, M-J., et al. 2007. Journal of Biological Chemistry 282, 14253.
    • Ames, J.B., et al. 2006. Journal of Biological Chemistry 281, 37237.
    • Beloqui, A., et al. 2006. Journal of Biological Chemistry 281, 22933.
    • Pimentel, C., et al. 2006. Protein Science 15, 628.
    • Baneres, J-L., et al. 2005. Journal of Biological Chemistry 280, 20253.
    • Thai, K., et al. 2005. Protein Science 14, 948.
    • Juan Carlos Rodriguez, Lilly Wong and Patricia A. Jennings. 2003. Protein Expression and Purification 28, 224.
    • ユーザープロトコール

      タイトル
      TB055 pET System Manual
      TB104 pET Peptide Expression System 31

      ベクターマップ

      タイトル
      TB098VM pET-31b(+) Vector Map

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      カテゴリー

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors