87748
3,3′,5,5′-テトラメチルベンジジン
TMB substrate, chromogenic, ≥98.0% (NT), powder
別名:
BM ブルー, Sure Blue TMB, TMB, TMB ブロッティングプラス, TMB 基質
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この商品について
化学式:
[-C6H2(CH3)2-4-NH2]2
CAS番号:
分子量:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
Beilstein/REAXYS Number:
2808541
製品名
3,3′,5,5′-テトラメチルベンジジン, ≥98.0% (NT)
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
assay
≥98.0% (NT)
form
powder
technique(s)
UV/Vis spectroscopy: suitable
mp
166-171 °C
168-171 °C (lit.)
storage temp.
2-8°C
Quality Level
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Application
3,3′,5,5′テトラメチルベンジジン（TMB）は、ベンジジンの非発癌性代替物（Ames試験陰性）です。ELISAで使用するペルオキシダーゼ基質として適しています。淡い青色の可溶性最終生成物を生成し、370または620～650 nmで分光光度計を用いて読み取ることができます。TMB反応は2 M H2SO4を用いて停止することができ（結果は黄色になります）、450 nmで読み取ります。血液を検出する ヘモグロビンアッセイ、ペルオキシダーゼアッセイ用の高感度で特異的な試薬です。
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
- enzyme-immunoassay (EIA)
- indirect competitive enzyme-linked immunosorbent assay (ELISA)
- sandwich enzyme-linked immunosorbent assay
flash_point_c
Not applicable
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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