M8823
抗-FLAG® M2磁性ビーズ
affinity isolated antibody
別名:
モノクローナル抗FLAG® M2抗体 マウス宿主抗体, FLAG®アフィニティーレジン, FLAG®レジン(ハイスループット), FLAG®磁気アフィニティーレジン, 抗ddddk, 抗dykddddk
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この商品について
conjugate
magnetic beads
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
suspension
shelf life
2 yr at -20 °C
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
bead size
20-75 μm
matrix
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
isotype
IgG1
capacity
≥0.6 mg/mL binding capacity
shipped in
wet ice
storage temp.
−20°C
General description
Application
Features and Benefits
－きわめて迅速な分離
－洗浄の繰り返しなど、操作が大幅に加速
－プレートフォーマットで複数サンプルの処理を実施
これにより、以下が実現します。
－実験の加速
－再現性の改善
－関心タンパク質の定量精度の向上
Physical form
Legal Information
Disclaimer
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保管分類
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
資料
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
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