R1158
Ribonuclease Inhibitor
recombinant, expressed in E. coli, 20-40 units/μL
別名:
RNAse inhibitor
Application
Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:
- cDNA synthesis from mRNA
- reverse transcriptase polymerase chain reaction (RT-PCR)
- in vitro transcription and translation
- mRNA protection from degradation
- formaldehyde cross-linked lncRNA purification
- RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Biochem/physiol Actions
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
General description
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).
Other Notes
- Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
- This product is for R&D use only, not for drug, household, or other uses.
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Physical form
Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.
保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Comparison of in vitro viability methods for Cryptosporidium oocysts
Burgt NHV, et al.
Experimental Parasitology, 187, 30-36 (2018)
Nathan H Vande Burgt et al.
Experimental parasitology, 187, 30-36 (2018-03-09)
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of
Nadia A Hasaneen et al.
American journal of physiology. Lung cellular and molecular physiology, 293(4), L1059-L1068 (2007-08-19)
Angiogenesis is an important feature of airway remodeling in both chronic asthma and chronic obstructive pulmonary disease (COPD). Airways in those conditions are exposed to excessive mechanical strain during periods of acute exacerbations. We recently reported that mechanical strain of
Lintao Li et al.
Cell cycle (Georgetown, Tex.), 22(4), 476-493 (2022-11-13)
Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected to microCT analysis
A fine-needle aspiration-based protein signature discriminates benign from malignant breast lesions
Franzen B, et al.
Molecular Oncology, 12(9), 1415-1428 (2018)
資料
mRNAワクチンがどのように免疫を誘導するのかを理解し、ワクチン免疫原やバイオ医薬品用に合成mRNAがどのように調製されるのかご覧ください。mRNA合成用試薬についてご紹介します。
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
関連コンテンツ
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
Instructions
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