- Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
BMC genomics (2014-11-02)
Huijun Cheng, Xuewen Xu, Tracy Hadfield, Noelle Cockett, Carole Charlier, Michel Georges, Haruko Takeda
PMID25359221
ABSTRACT
Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
MATERIALS
Product Number
Brand
Product Description
MOPS, BioUltra, Molecular Biology, ≥99.5% (titration)
BIS-TRIS, BioUltra, ≥99.0% (NT)
MOPS, BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)
MOPS, BioXtra, ≥99.5% (titration)
MOPS, ≥99.5% (titration)
BIS-TRIS, BioXtra, ≥98.0% (titration)
BIS-TRIS, ≥98.0% (titration)
BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
MOPS, anhydrous, free-flowing, Redi-Dri™, ≥99.5%
Potassium phosphate monobasic, 99.99% trace metals basis
Potassium phosphate monobasic, Molecular Biology, ≥98.0%
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Potassium phosphate monobasic, ReagentPlus®
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)
Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder
Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
Ethylenediaminetetraacetic acid, ≥98.0% (KT)
Potassium phosphate monobasic, tested according to Ph. Eur., anhydrous
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Ethylenediaminetetraacetic acid, 99.995% trace metals basis
Monobasic Potassium Phosphate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
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Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, pH 8.0, ~0.5 M in H2O
Potassium phosphate monobasic, meets analytical specification of Ph. Eur., NF, E340, anhydrous, 98-100.5% (calc. to the dried substance)
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Potassium phosphate monobasic, buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%