AY-27 Rat Bladder Tumor Cell Line

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Bladder cancer is one of the top ten most common malignancies worldwide, and no cure is available for metastatic stages of the disease (1). Bladder cancer generally arises from the inner epithelial layer (urothelium) (1). A suitable bladder tumor model that recapitulates human disease both histologically and behaviorally is essential for evaluating new therapeutic agents and modes of treatment. Rat models more closely parallel human bladder carcinoma than mouse models regarding configuration and progress of the disease (2).

The AY-27 rat bladder tumor cell line is a urothelium-derived transitional cell carcinoma (3) and is the most widely used animal model for bladder cancer. AY-27 cells are tumorigenic and are characterized by low adenylate cyclase activity (4), lack of FasL expression, and positive staining for the urothelial marker cytokeratin-7.5 Tumors derived from AY-27 cells demonstrate orthotopic growth, allowing exposure to anti-tumor drugs in a natural environment (5). The high reproducibility and clinical relevance of the AY-27 tumor model has contributed greatly to the understanding of bladder cancer and evaluation of potential treatments.

References:
1. Sanli O et al. (2017) Bladder cancer. Nat Rev Dis Primers 3: 17022.
2. Oyasu R (1995) Epithelial tumors of the lower urinary tract in humans and rodents. Food Chem Toxicol. 33(9): 747-755.
3. Cohen SM et al. (1981) Transplantation and cell culture of rat urinary bladder carcinoma. Invest Urol. 19(3): 136-141.
4. Chlapowski FJ, Nemecek GM (1985) Aberrant cyclic adenosine 3′:5′-monophosphate metabolism in cultures of tumorigenic rat urothelium. Cancer Res. 45(1): 122-127.
5. Xiao Z et al. (1999) Characterization of a novel transplantable orthotopic rat bladder transitional cell tumor model. Br J Cancer 81(4): 638-646.

Source: The AY-27 cell line was established from a primary bladder tumor induced by N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) treatment of Fischer F344 rats (2).

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.