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CP01 Anti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Anti-Actin (Ab-1) Mouse mAb (JLA20) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP01
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

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      Description
      OverviewRecognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
    • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
    • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
    • Entrez Gene Name: actin, alpha 1, skeletal muscle
    • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
    • Mu Entrez ID: 11459 ;-; 11465
    • Rat Entrez ID: 29437 ;-; 287876
      		•
      Catalogue NumberCP01
      Brand Family Calbiochem®
      References
      ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
      Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
      Product Information
      FormLiquid
      Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
      Positive controlAll animal cell lines
      Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (1:5000-1:20000)
      Immunofluorescence (1:10000, see application references)

      Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
      Application CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenchicken cytoskeletal proteins
      ImmunogenChicken
      CloneJLA20
      HostMouse
      IsotypeIgM
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP01-1EACN 07790788053697

      Documentation

      Anti-Actin (Ab-1) Mouse mAb (JLA20) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Actin (Ab-1) Mouse mAb (JLA20) Certificates of Analysis

      TitleLot Number
      CP01

      References

      Reference overview
      Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
      Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

      Brochure

      Title
      Antibody Sourcebookﾙ Sixth Edition

      Citations

      Title
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2008 JSW
      ApplicationImmunoblotting (1:5000-1:20000)
      Immunofluorescence (1:10000, see application references)

      Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
      DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
      BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
      HostMouse
      Immunogen speciesChicken
      Immunogenchicken cytoskeletal proteins
      CloneJLA20
      IsotypeIgM
      Speciesa broad range of species
      Positive controlAll animal cell lines
      FormLiquid
      Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
      Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
      CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
      Toxicity Multiple Toxicity Values, refer to MSDS
      ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
      Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
      Citation
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
      		•
      Application referencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.