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MAB4132-25UG Anti-BRCA-1, clone BR64

Anti-BRCA1 Antibody, clone BR64 is a high quality Mouse Monoclonal Antibody for the detection of BRCA1 & has been validated in IP & WB.

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MAB4132-25UG
25 μg  
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      Description
      Catalogue NumberMAB4132-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BRCA-1, clone BR64
      References
      Product Information
      FormatPurified
      PresentationLiquid in 0.02M phosphate buffer, 0.25M NaCl, containing 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BRCA1 Antibody, clone BR64 is a high quality Mouse Monoclonal Antibody for the detection of BRCA1 & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation

      Western blot

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified polypeptide representing a 100-amino acid segment of the amino terminus of the BRCA1 gene product.
      CloneBR64
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe breast cancer susceptibility gene, BRCA1*, is located on chromosome 17 (at q21), and is mutated in the majority of heritable breast cancers. The mutations within the coding region occur throughout the gene with little apparent clustering. The majority of BRCA1 mutations result in shortened BRCA1 gene products, creating a need for an amino-terminal marker. Antibody MAB4132 recognizes both the full-length and mutated (shortened) forms of BRCA1 in breast cancer cell lines. It also recognizes over-expressed protein in transiently transfected Cos cells. A single cross-reacting protein of approximately 27 kDa is also seen upon immunoprecipitation. No other cross-reacting proteins are detected.

      BRAC1 is 220kDa.

      *BRCA1 is covered under U.S. patents 5,747,282, 5,753,441 and 6,162,897.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
      Gene Symbol
      • BRCA1
      • RNF53
      • BRCAI
      • PSCP
      • BRCC1
      • IRIS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P38398 # Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Mediates E2-dependent ubiquitination. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
      SIZE: 1863 amino acids; 207721 Da
      SUBUNIT: Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. CTIP interacts specifically with the BRCT domains. Interacts with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Binds BRIP1 through the BRCT domains. Interacts with ubiquitinated FANCD2. Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts through its BRCT domains with phosphorylated ACACA and prevents its dephosphorylation.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P38398 The RING-type zinc finger domain interacts with BAP1.
      PTM: Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
      DISEASE: "SwissProt: P38398 # Defects in BRCA1 are a cause of genetic susceptibility to breast cancer (BC) [MIM:113705, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination. & Defects in BRCA1 are a cause of genetic susceptibility to breast-ovarian cancer (BOC) [MIM:113705]. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast- ovarian cancer. & Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705]."
      SIMILARITY: Contains 2 BRCT domains. & Contains 1 RING-type zinc finger.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4132-25UG 04054839350382

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