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MABT1454-100UL Anti-Claudin-2 Antibody, clone 1A2

Anti-Claudin-2, clone 1A2, Cat. No. MABT1454, is a rat monoclonal antibody that detects Claudin-2 and is tested for use in Flow Cytometry and Inhibition Assays.

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MABT1454-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1454-100UL
      DescriptionAnti-Claudin-2 Antibody, clone 1A2
      Alternate Names
      • SP82
      Background InformationClaudin-2 (UniProt: P57739; also known as SP82) is encoded by the CLDN2 (also known as PSEC0059, SP82, UNQ705/PRO1356) gene (Gene ID: 9075) in human. Claudin-2 is a multi-pass membrane protein with three cytoplasmic domains, two extracellular domains, and four transmembrane domains. It can exist as homopolymer or as a heteropolymer with other claudins mediated through the first extracellular domain (aa 29-81). These interactions are achieved largely through electrostatic interactions with Asp65 and with somewhat weaker interaction with electrons of the Tyr67. Claudin-2 is expression has been reported in liver, kidney, and small intestine. It is a component of epithelial tight junctions (TJ) that plays a key role in TJ-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Its expression is shown to be enhanced in subjects with inflammatory bowel disease. Treatment with TNF-a is also reported to upregulate the expression of Claudin-2 via PI3-kinase signaling that reduces transepithelial electrical resistance (TEER). Clone 1A2 is shown to reduce TNF-a-induced reduction in TEER and restore TJ integrity. (Ref.: Takigawa, M., et al. (2017). J. Pharmacol. Exp. Ther. 363(3); 444-451).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Claudin-2, clone 1A2, Cat. No. MABT1454, is a rat monoclonal antibody that detects Claudin-2 and is tested for use in Flow Cytometry and Inhibition Assays.
      Key Applications
      • Flow Cytometry
      • Inhibition
      Application NotesFlow Cytometry Analysis: A representative lot detected Claudin-2 in Flow Cytometry applications (Takigawa, M., et. al. (2017). J Pharmacol Exp Ther. 363(3):444-451).

      Inhibition Assay: A representative lot of this antibody inhibited TNFa-induced dysfunction of tight junction seals in the intestinal epithelium. (Takigawa, M., et. al. (2017). J Pharmacol Exp Ther. 363(3):444-451).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length human recombinant Claudin-2.
      EpitopeExtracellular domain
      Clone1A2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 1A2 is a rat monoclonal antibody that detects Claudin-2. It targets an epitope within the first extracellular loop.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLDN2
      • PSEC0059
      • SP82
      • UNQ705/PRO1356
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight24.55 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HT1080 cells transfected with human Claudin-2.

      Flow Cytometry Analysis (FC): 1 µg of this antibody detected Claudin-2 in one million HT1080 cells transfected with human Claudin-2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1454-100UL 04065265944809