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MABT1457-100UG Anti-Collagen Antibody, Type VII Antibody, clone LH24

Anti-Collagen, Type VII, clone LH24, Cat. No. MABT1457, is a mouse monoclonal antibody that detects human Collagen type VII and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABT1457-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1457-100UG
      DescriptionAnti-Collagen Antibody, Type VII Antibody, clone LH24
      Alternate Names
      • Collagen alpha-1(VII) chain
      • Long-chain collagen
      • LC collagen
      Background InformationCollagen alpha-1(VII) chain (UniProt: Q02388; also known as Long-chain collagen, LC collagen) is encoded by the COL7A1 gene (Gene ID: 1294) in human. LH24 targets the basement membrane zone of keratinocytes, specifically Type VII Collagen non-collagenous domain 2. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagen with at least 38 distinct polypeptide chains. Collagen type VII is primarily synthesized by keratinocytes and skin fibroblasts. It is composed of three identical alpha chains and its distribution is restricted to stratified squamous epithelial basement membrane. It contributes to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins. Type VII collagen contains a non-collagenous domain 1 (NC1), a central collagenous domain, and a non-collagenous domain 2 (NC2) that catalyze the anchoring fibril assembly. Mutations in COL7A1 gene have been linked to epidermolysis bullosa dystrophica, a blistering skin disease characterized by tissue separation that occurs below the dermal-epidermal basement membrane at the level of the anchoring fibrils. (Ref.: Siprashvili, Z., et al. (2016). J. Am. Med. Assoc. 316(17); 1808-1817; Chung, HJ., and Uitto, J (2010). Dermatol. Clin. 28(1); 93-105).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Collagen, Type VII, clone LH24, Cat. No. MABT1457, is a mouse monoclonal antibody that detects human Collagen type VII and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunohistochemistry
      • Electron Microscopy
      Application NotesImmunohistochemistry Applications: A representative lot detected Collagen, Type VII, in Immunohistochemistry applications (Woodley, D.T., et. al. (2017). J Clin Invest. 127(8):3028-3038; Siprashvili, Z., et. al. (2016). JAMA. 316(17):1808-1817).

      Western Blotting Analysis: A representative lot detected Collagen, Type VII, in Western Blotting applications (Siprashvili, Z., et. al. (2016). JAMA. 316(17):1808-1817).

      Electron Microscopy: A representative lot detected Collagen, Type VII, in Electron Microscopy applications (Twaroski, K., et. al. (2019). Br J Dermatol. 181(6):1247-1253).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenCrude extract from human skin.
      EpitopeC-terminal
      CloneLH24
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone LH24 is a mouse monoclonal antibody that detects human Collagen alpha-1(VII) chain. It targets an epitope within the C-terminal noncollagenous domain (NC2).
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COL7A1
      Purification MethodLigatrap
      UniProt Number
      Molecular Weight295.22 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunofluorescence in frozen human skin tissue sections.

      Immunofluorescence Analysis: A 1:25 dilution of this antibody detected Collagen, Type VII in frozen human skin tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1457-100UG 04065265397971

      Documentation

      Anti-Collagen Antibody, Type VII Antibody, clone LH24 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Collagen Antibody, Type VII Antibody, clone LH24 Certificates of Analysis

      TitleLot Number
      Anti-Collagen, Type VII, clone LH24 - 4010110 4010110
      Anti-Collagen, Type VII, clone LH24 - 4021517 4021517
      Anti-Collagen, Type VII, clone LH24 - 4027401 4027401
      Anti-Collagen, Type VII, clone LH24 - 4027808 4027808
      Anti-Collagen, Type VII, clone LH24 - 4033094 4033094
      Anti-Collagen, Type VII, clone LH24 - 4044228 4044228
      Anti-Collagen, Type VII, clone LH24 - 4044762 4044762
      Anti-Collagen, Type VII, clone LH24 - 4086386 4086386
      Anti-Collagen, Type VII, clone LH24 - 4148787 4148787
      Anti-Collagen, Type VII, clone LH24 - 4186015 4186015
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