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MABS2226-25UL Anti-ELOVL3 Antibody, clone V61 P2B3 D10

Anti-ELOVL3, clone V61 P2B3 D10, Cat. No. MABS2226, is a mouse monoclonal antibody that detects ELOVL3 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

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MABS2226-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2226-25UL
      DescriptionAnti-ELOVL3 Antibody, clone V61 P2B3 D10
      Alternate Names
      • Elongation of very long chain fatty acids protein 3
      • EC:2.3.1.199
      • 3-keto acyl-CoA synthase ELOVL3
      • Cold-inducible glycoprotein of 30 kDa
      • ELOVL fatty acid elongase 3
      • ELOVL FA elongase 3
      • Very long chain 3-ketoacyl-CoA synthase 3
      • Very long chain 3-oxoacyl-CoA synthase 3
      Background InformationElongation of very long chain fatty acids protein 3 (UniProt: Q9HB03; also known as EC:2.3.1.199, 3-keto acyl-CoA synthase ELOVL3, Cold-inducible glycoprotein of 30 kDa, ELOVL fatty acid elongase 3, ELOVL FA elongase 3, Very long chain 3-ketoacyl-CoA synthase 3, Very long chain 3-oxoacyl-CoA synthase 3) is encoded by the ELOVL3 (also known as CIG30) gene (Gene ID: 83401) in human. ELOVL3 is a multi-pass membrane protein of endoplasmic reticulum (ER). It catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. Its C-terminal di-lysine motif (aa 266-270) confers endoplasmic reticulum localization. This ER-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. It exhibits higher activity towards C18:0 acyl-CoAs. It is strongly expressed in normal skin, breast, colon, liver tissues. A decrease in the expression of ELOVL3 is reported in colorectal tumors compared to normal colorectal epithelium. ELOVL3 is reported to be involved in the regulation of the progression of adipogenesis through activation of peroxisome proliferator-activated receptor (PPAR) in mouse adipocytic 3T3-L1 cells. Its levels are elevated more than200-fold in cold-stressed animals. (Ref.: Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147; Kobayashi, T., and Fujimori, K. (2012). 302(12); E1461-E1471; Westerberg, R., et al. (2006). J. Biol. Chem. 281(8); 4958-4968).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ELOVL3, clone V61 P2B3 D10, Cat. No. MABS2226, is a mouse monoclonal antibody that detects ELOVL3 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • ELISA
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected ELOVL3 in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 45(4):1138-1147).

      ELISA Analysis: A representative lot detected ELOVL3 in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

      Immunohistochemistry Applications: A representative lot detected ELOVL3 in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 9 amino acids from the N-terminal region of human Elongation of very long chain fatty acids protein 3 (ELOVL3).
      EpitopeN-terminal
      CloneV61 P2B3 D10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone V61 P2B3 D10 is a mouse monoclonal antibody that detects Elongation of very long chain fatty acids protein 3 (ELOVL3). It targets and epitope within 9 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ELOVL3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight31.5 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human colon cancer tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected ELOVL3 in human colon cancer tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2226-25UL 04065268041840