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MAB2626 Anti-Endosialin Antibody, clone B1/35

Detect Endosialin using this Anti-Endosialin Antibody, clone B1/35 validated for use in WB, IP, IF, FC.

Anti-Endosialin Antibody, clone B1/35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: CD248, TEM1, CD164L1 UniProt Number: Q9HCU0
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MAB2626
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, IF, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2626
      DescriptionAnti-Endosialin Antibody, clone B1/35
      Alternate Names
      • CD248 molecule, endosialin
      • CD164 sialomucin-like 1
      • endosialin
      • CD248 antigen, endosialin
      • Tumor endothelial marker 1
      • CD248 antigen
      Background InformationEndosialin is a glycoprotein on the cell surface of vascular endothelial cells in a broad range of human cancers. Endosialin is thought to be highly expressed at the points of active tissue remodeling and neovascularisation which may imply that endosialin is key in tumor growth, angiogenesis and metastasis. Endosialin has structural similarity to CD93, which involves a C-type lectin-like domain, EGF-like repeats and a glycosylated mucin-like domain. This similarity may also suggest that endosialin plays a role in innate immunity and inflammation.
      References
      Product Information
      FormatPurified
      Control
      • AG1523 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Endosialin using this Anti-Endosialin Antibody, clone B1/35 validated for use in WB, IP, IF, FC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect Endosialin in rheumatoid fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

      Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP on AG1523 human fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

      Immunofluorescent Analysis: A previous lot was used by an independent laboratory in IF to detect Endosialin in human rheumatoid synovium, tonsil, salivary gland and normal breast tissues (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).
      Biological Information
      ImmunogenDiploid human fibroblasts
      CloneB1/35
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD248
      • TEM1
      • CD164L1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May play a role in tumor angiogenesis.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable.

      TISSUE SPECIFICTY: Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.

      PTM: O-glycosylated with sialylated oligosaccharides.

      May be N-glycosylated.

      SEQUENCE SIMILARITIES: Contains 1 C-type lectin domain.

      Contains 1 EGF-like domain.

      Contains 1 Sushi (CCP/SCR) domain.
      Molecular Weight~175 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in AG1523 cell lysate.

      Western Blot Analysis: A 1:2,500 dilution of this antibody detected Endosialin in 10 µg of AG1523 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2626 04053252482458

      Documentation

      Anti-Endosialin Antibody, clone B1/35 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Endosialin Antibody, clone B1/35 Certificates of Analysis

      TitleLot Number
      Anti-Endosialin, clone B1/35 2475034
      Anti-Endosialin, clone B1/35 - 1953015 1953015
      Anti-Endosialin, clone B1/35 - 1987097 1987097
      Anti-Endosialin, clone B1/35 - 2009453 2009453
      Anti-Endosialin, clone B1/35 - 2043215 2043215
      Anti-Endosialin, clone B1/35 - 2049281 2049281
      Anti-Endosialin, clone B1/35 - 2211068 2211068
      Anti-Endosialin, clone B1/35 - 3388679 3388679
      Anti-Endosialin, clone B1/35 - 3862690 3862690
      Anti-Endosialin, clone B1/35 - 3900078 3900078
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