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ABC1469 Anti-NRP1

Anti-NRP1, Cat. No. ABC1469, is a rabbit polyclonal antibody that detects human Neuropilin-1 and has been tested for use in Immunohistochemistry (Paraffin), Inhibition Activity/Function, and Western Blotting.

Anti-NRP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: NRP1, NRP, VEGF165R UniProt Number: O14786
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ABC1469
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), INHIB, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1469
      DescriptionAnti-NRP1
      Alternate Names
      • Vascular endothelial cell growth factor 165 receptor
      • CD304
      Background InformationNeuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Three isoforms of Neuropilin-1 have been reported that are produced by alternative splicing. Isoform 1 of Neuropilin-1 is a homodimer, single-pass type I membrane protein with three calcium-binding sites (aa 195, 209, and 250). It can also form heterodimers with neuropilin-2. This membrane-bound isoform serves as a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF165 isoform of VEGFA and VEGFB. Its co-expression with VEGFR-2 (KDR) results in increased VEGF165 binding to VEGFR-2 as well as increased chemotaxis. It has an extracellular domain (aa 22-856), a helical domain (aa 857-879), and the cytoplasmic domain (aa 880-923). It is produced with a signal peptide (aa 1-21) that is subsequently cleaved to generate the mature form. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. It may also bind various members of the semaphorin family and its expression has an adverse effect on blood vessel number and integrity. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NRP1, Cat. No. ABC1469, is a rabbit polyclonal antibody that detects human Neuropilin-1 and has been tested for use in Immunohistochemistry (Paraffin), Inhibition Activity/Function, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Inhibits Activity/Function
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected NRP1 in human prostate cancer and human bone marrow tissue.

      Western Blotting Analysis: A representative lot detected NRP1 in Western Blotting applications (Pang, H.B., et. al. (2014). Nat Commun. 5:4904).

      Inhibits Activity/Function: A representative lot reduced iRGD-induced vascular permeability in tumor tissue and reduced the uptake of nanoparticles. (Pang, H.B., et. al. (2015). Sci Adv. 1(10):e1500821; Sugahara, K.N., et. al. (2010). Science. 328(5981):1031-5).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 313 amino acids from the internal region of human Neuropilin-1.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Neuropilin-1 in human cells. It targets an epitope within 313 amino acids from the internal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NRP1
      • NRP
      • VEGF165R
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~36 kDa observed for recombinant fragment. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Neuropilin-1.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected recombinant Neuropilin-1. recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1469 04054839383656