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ABS533 Anti-PTEN mono-ubiquitylated K13

Anti-PTEN mono-ubiquitylated K13, Cat. No. ABS533, is a rabbit polyclonal antibody that detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-PTEN mono-ubiquitylated K13 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PTEN, MMAC1, TEP1 UniProt Number: P60484
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ABS533
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS533
      DescriptionAnti-PTEN mono-ubiquitylated K13
      Alternate Names
      • Phosphatidylinositol 3
      • 4
      • 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
      • Mutated in multiple advanced cancers 1
      • Phosphatase and tensin homolog
      • Phosphatase and tensin homologue deleted on chromosome 10
      Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. Lysine 13 is one of the conserved sites for ubiquitination and monoubiquitination is essential for the movement of PTEN from cytoplasm to the nucleus. NEDD4-1 is identified as E3 ligase that induces both mono- and poly-ubiquitination of PTEN.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PTEN mono-ubiquitylated K13, Cat. No. ABS533, is a rabbit polyclonal antibody that detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:1500 dilution from a representative lot blocked PTEN mono-ubiquitinated K13 in HEK293 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding ubiquitinated Lysine 13 from the N-terminal region of human PTEN.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN monoubiquitinated on Lysine 13. Immunogen sequence is conserved, hence, it is expected to react with all 3 isoforms.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTEN
      • MMAC1
      • TEP1
      Modifications
      • Ubiquitylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~65 kDa observed; 47.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected PTEN mono-ubiquitinated K13 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS533 04054839367144