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MABF2789-25UL Anti-SARS-CoV-2 nucleocapsid Antibody, clone 5B9-C6

Anti-SARS-CoV-2 nucleocapsid, clone 5B9-C6, Cat. No. MABF2789, is a mouse monoclonal antibody that detects Nucleoprotein in SARS-CoV-2 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

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MABF2789-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2789-25UL
      DescriptionAnti-SARS-CoV-2 nucleocapsid Antibody, clone 5B9-C6
      Alternate Names
      • SARS-CoV-2 Nucleoprotein
      • Nucleocapsid protein
      • SARS-CoV-2 NC
      • Protein N
      Background InformationSARS-CoV-2 Nucleoprotein (UniProt: P0DTC9; also known as N, Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 43740575) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. The N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. The RNA binding region of SARS-CoV-2 is localized to amino acids 41-186 and its dimerization domain is in amino acids 258-361. Lack of cysteine is one of the common features in nucleoprotein from different coronaviruses. The complete N protein of SARS-CoV and COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines and threonines and some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. Clone 5B9-C6 is specific for SARS-CoV-2 and does not react with SARS-CoV-1.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV-2 nucleocapsid, clone 5B9-C6, Cat. No. MABF2789, is a mouse monoclonal antibody that detects Nucleoprotein in SARS-CoV-2 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunofluorescence
      Application NotesTested Applications

      Immunoprecipitation Analysis: A representative lot Immunoprecipitated SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      ELISA Analysis: A 1:3 dilution from a representative lot detected SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Western Blotting Analysis: A 1:1,000 dilution from a represenative lot detected SARS-CoV-2 nucleocapsid in Whole cell lysates of HEK293T cells expressing the nucleocapsid (NC) proteins of various human coronaviruses (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Immunofluorescence Analysis: A 1:100 dilution from a represenative lot detected SARS-CoV-2 nucleocapsid in HeLa cells transiently transfected with SARS-CoV-2 NC-P2A-GFP (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length nucleocapsid protein from Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
      EpitopeN-terminal
      Clone5B9-C6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5B9-C6 is a mouse monoclonal antibody that detects nucleocapsid protein of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It targets an epitope within the N-terminal domain. It does not cross react with SARS virus.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteSARS-CoV-2
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~53 kDa observed; 45.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Nucleocapsid protein in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein, but not in lysates from the wild type HEK293 T cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2789-25UL 04065266553840