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MABN841 Anti-Semaphorin 4A Antibody, clone 8B5.1

This Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.

Anti-Semaphorin 4A Antibody, clone 8B5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SEMA4A UniProt Number: Q9H3S1
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MABN841
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN841
      DescriptionAnti-Semaphorin 4A Antibody, clone 8B5.1
      Alternate Names
      • Semaphorin 4A
      • Semaphorin-B
      • Sema B
      • Sema4A
      Background InformationSemaphorin-4A (UniProt Q9H3S1; also known as Sema B, Semaphorin-B) is encoded by the SEMA4A (also known as CORD10, RP35, SEMAB, SEMB) gene (ORP UNQ783/PRO1317; Gene ID 64218) in human. Semaphorin-4A is a membrane-type class IV semaphorin originally characterized for its role in axon guidance during neural development, Semaphorin-4A also plays a role in Th cells activation and the differentiation of Th1 and Th17 cells. In addition Semaphorin-4A is involved in angiogenesis and endothelial cells migration by modulating VEGF signaling. Consistent with its important role in Th cells, Semaphorin-4A level is reported to be elevated in the serum samples from patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Semaphorin-4A neutralizing antibodies are reported to inhibit antigen-specific T cell generation and ameliorate experimental autoimmune encephalomyelitis (EAE), an animal model of MS.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected Semaphorin 4A in 10 µg of human retinal pigment epithelia (RPE) ARPE-19.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the Sema domain of human Semaphorin 4A.
      EpitopeSema domain
      Clone8B5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SEMA4A
      UniProt Number
      Molecular Weight~85 kDa observed. 83.6 kDa (isoform 1) and 69.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human monocytic leukemia THP-1 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4A in 10 µg of human monocytic leukemia THP-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN841 04055977277005