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MABT523 Anti-beta-Actin Antibody, clone RM112

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Anti-beta-Actin Antibody, clone RM112 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ACTB UniProt Number: P60709
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MABT523
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT523
      DescriptionAnti-beta-Actin Antibody, clone RM112
      Alternate Names
      • Actin
      • cytoplasmic 1
      • Beta-actin
      • beta-Actin
      Background InformationActin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show > 90% amino-acid (aa) homology between isotypes and > 98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
      Biological Information
      ImmunogenA peptide corresponding to the N-terminus of beta-Actin.
      EpitopeN-terminus
      CloneRM112
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ACTB
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT523 04055977181555

      Documentation

      Anti-beta-Actin Antibody, clone RM112 Certificates of Analysis

      TitleLot Number
      Anti-beta-Actin, clone RM112 -VP1606030 VP1606030
      Anti-beta-Actin, clone RM112 - VP1907262 VP1907262
      Anti-beta-Actin, clone RM112 - VP2001075 VP2001075
      Anti-beta-Actin, clone RM112 - VP2008070 VP2008070
      Anti-beta-Actin, clone RM112 - VP2106251 VP2106251
      Anti-beta-Actin, clone RM112 - VP2109163 VP2109163
      Anti-beta-Actin, clone RM112 - VP2111182 VP2111182
      Anti-beta-Actin, clone RM112 - VP2201262 VP2201262
      Anti-beta-Actin, clone RM112 - VP2209020 VP2209020
      Anti-beta-Actin, clone RM112 - VP2310035 VP2310035
      Show more

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies