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MABS2313-25UG Anti-iRhom1 Antibody, clone RHF1A 20A8

Anti-iRhom1, clone RHF1A 20A8, Cat. No. MABS2313, is a rat monoclonal antibody that detects iRhom1 and is tested for use in Western Blotting.

MABS2313-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2313-25UG
      DescriptionAnti-iRhom1 Antibody, clone RHF1A 20A8
      Alternate Names
      • Inactive rhomboid protein 1
      • Epidermal growth factor receptor-related protein
      • Rhomboid 5 homolog 1
      • Rhomboid family member 1
      • p100hRho
      Background InformationInactive rhomboid protein 1 (UniProt: Q96CC6; also known as iRhom1, Epidermal growth factor receptor-related protein, Rhomboid 5 homolog 1, Rhomboid family member 1, p100hRho, RHBDF1) is encoded by the RHBDF1 (also known as C16orf8, DIST1, IRHOM1) gene (Gene ID: 64285) in human. iRhom1 is a multi-pass, homodimeric, membrane protein that is predominantly localized in the endoplasmic reticulum (ER). It is ubiquitously expressed, but higher expression is observed in brain. It regulates the maturation and activity of ADAM17, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF . It also controls the transport of ADAM17 from ER to the plasma membrane and removal of its inhibitory propeptide, and stability and degradation. It is reported to play a role in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own due to lack of amino acids that are required for proteolytic activity. iRhom1-deficient mice are reported to lack mature ADAM17 in the brain. (Ref.: Tüshaus, J., et al. (2021). FASEB J. 35(11); e21962; Weskamp, G., et al. (2020). J. Biol. Chem. 295(23); 4350-4358).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-iRhom1, clone RHF1A 20A8, Cat. No. MABS2313, is a rat monoclonal antibody that detects iRhom1 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      • Western Blot Using KO Lysates
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected iRhom1 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected iRhom1 in Mouse embryonic fibroblasts (MEF) lysates, but not in lysates with iRhom1 knockout (Courtesy of Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich, Germany).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Inactive rhomboid protein 1 (iRhom1).
      EpitopeN-terminal
      CloneRHF1A 20A8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone RHF1A 20A8 is a rat monoclonal antibody that detects Inactive rhomboid protein 1 (iRhom1). It targets an epitope within 19 amino acids from the N-terminal region.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RHBDF1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~97 kDa observed; 97.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 721.221 cell lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected iRhom1 in 721.221 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2313-25UG 04065270938077