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ABE1453 Anti-phospho Brd4 (Ser492/Ser494)

Anti-phospho Brd4 (Ser492/Ser494), Cat. No. ABE1453, is a highly specific rabbit polyclonal antibody that targets Bromodomain-containing protein 4 and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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ABE1453
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1453
      DescriptionAnti-phospho Brd4 (Ser492/Ser494)
      Alternate Names
      • Bromodomain-containing protein 4
      • Protein HUNK1
      Background InformationBromodomain-containing protein 4 (UniProt O60885; also known as MCAP, Mitotic chromosome-associated protein, Protein HUNK1) is encoded by the BRD4 (also known as HUNK1, MCAP) gene (Gene ID 23476) in human. Bromodomain-containing protein 4 is a BET (bromodomain and extra-terminal) protein family member that functions as a chromatin reader by binding acetylated lysines in histones. It plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. It remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post-mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, it plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Bromodomain-containing protein 4 is required to form the transcriptionally active P-TEFb complex where it displaces negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb and transforms it into an active form that can then phosphorylate the C-terminal domain of RNA polymerase II. It promotes the phosphorylation of Ser2 of the C-terminal domain of RNA polymerase II. In addition to acetylated histones, Bromodomain-containing protein 4 also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. It is also reported to act as a regulator of p53/TP53-mediated transcription and following phosphorylation by casein kinase 2K2, it is recruited to p53/TP53 specific target promoters. BRD4 knockout in mice is shown to be lethal, while BRD4 function blockage by inhibitor JQ1 (Cat. No. 500586) treatment is reported to affect the transcription of critical synaptic proteins, resulting in memory deficits and decreased seizure susceptibility in mice.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho Brd4 (Ser492/Ser494), Cat. No. ABE1453, is a highly specific rabbit polyclonal antibody that targets Bromodomain-containing protein 4 and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Dot Blot
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in whole cell neuronal lysate (Courtesy of Erica Korb, Ph.D., Rockefeller University).

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in cultured cortical cells from mouse (C47blac6). (Courtesy of Erica Korb, Ph.D., Rockefeller University).

      Dot Blot Analysis: A representative lot detected phospho Brd4 (Ser492/Ser494) in phospho Brd4 peptides (Courtesy of Erica Korb, Ph.D., Rockefeller University).


      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids surrounding Ser492/Ser494 from the internal N-terminal half of human phospho Brd4 protein.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Bromodomain-conntaining protein 4 phosphorylated at Ser492/Ser494. It targets an epitope of 14 amino acids in the N-terminal half.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Porcine
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BRD4
      • HUNK1
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight200 kDa observed, 152.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in humans IPS cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho Brd44 (Ser492/Ser494) in 10 µg of human IPS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1453 04054839224249

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