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ABT1383 Anti-phospho-Keratin-18 Antibody (Ser52)

This rabbit polyclonal Anti-phospho-Keratin-18 (Ser52) Antibody, Cat. No. ABT1383 is validated for use in Western Blotting for the detection of Keratin-18 Ser52 phosphorylation.

Anti-phospho-Keratin-18 Antibody (Ser52) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: KRT18, CYK18, PIG46 UniProt Number: P05783
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ABT1383
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT1383
      DescriptionAnti-phospho-Keratin-18 Antibody (Ser52)
      Alternate Names
      • Keratin
      • type I cytoskeletal 18
      • Ser52-phosphorylated
      • Cell proliferation-inducing gene 46 protein
      • Ser52-phosphorylated
      • CK-18
      • Ser52-phosphorylated
      • Cytokeratin-18
      • Ser52-phosphorylated
      • K18
      • Ser52-phosphorylated
      • Keratin-18
      • Ser52-phosphorylated
      Background InformationKeratin, type I cytoskeletal 18 (UniProt P05783; also known as Cell proliferation-inducing gene 46 protein, CK-18, Cytokeratin-18, K18, Keratin-18) is encoded by the KRT18 (also known as CYK18, PIG46) gene (Gene ID 3875) in human. Cytokeratin-18 (Keratin-18 or K18) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. All epithelial cells typically express at least one type-I and one type-II keratin, which form noncovalent heteropolymers. Keratin 18 is a type I intermediate filament protein that pairs with keratin 8 (K8/K18) in simple epithelia and plays an important role in protecting cells from stress, injury, and apoptosis. K18 is the only type-I keratin in adult hepatocytes and the major type-I keratin in many colonic tumor cell lines.The structure and function of K8/18 are regulated by posttranslational modifications, such as glycosylation, ubiquitination, and phosphorylation, including K8 phosphorylation on Ser8, Ser23, Ser73, Ser431, and K18 phosphorylation on Ser33 and Ser52. K18 phosphorylation has been proposed as a progression marker for human chronic hepatitis B (CHB). Progressively higher Ser52 phosphorylation is reported in non-cirrhotic and cirrhotic CHB livers, and progressive increase of Ser33 and Ser52 phosphorylation correlates with the elevation of both histological lesions and enzymatic activities of alanine aminotransferase in non-cirrhotic CHB livers.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-phospho-Keratin-18 (Ser52) Antibody, Cat. No. ABT1383 is validated for use in Western Blotting for the detection of Keratin-18 Ser52 phosphorylation.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Keratin-18 target region sequence containing phosphorylated Ser52.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detected upregulated cellular Keratin-18 Ser52 phosphorylation upon phosphatase inhibitors treatment. The Ser52 numbering is based on keratin-18 sequence without the initiation Met1, which was removed posttranslationally.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Target phosphorylation site is not conserved in murine and rat species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT18
      • CYK18
      • PIG46
      Modifications
      • Phosphorylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~48 kDa observed. 47.93 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected upregulated Keratin-18 Ser52 phosphorylation in 10 µg lysate from phosphatase inhibitors Calyculin A- and Okadaic acid-treated HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT1383 04054839092060