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ABC1461-25UL Anti-phospho-c-Myc (Ser347)

Anti-phospho-c-Myc (Ser347), Cat. No. ABC1461, is a rabbit polyclonal antibody that detects c-Myc phosphorylated on serine 347 and is tested for use in Peptide Inhibition Assay and Western Blotting.

ABC1461-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1461-25UL
      DescriptionAnti-phospho-c-Myc (Ser347)
      Alternate Names
      • Myc proto-oncogene protein
      • Class E basic helix-loop-helix protein 39
      • bHLHe39
      • Proto-oncogene c-Myc
      • c-Myc
      • Transcription factor p64
      Background InformationMyc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. It regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. c-Myc is shown to be critically involved in the regulation of several growth promoting signaling pathways and is considered as an immediate early response gene that is downstream of many ligand-membrane receptor complexes. Its expression is reported to be tightly regulated by several transcriptional regulatory motifs within its proximal promoter region. Phosphorylation of c-Myc by serine/threonine kinase PIM2 at serine 329 is reported to lead to its stabilization. Active c-Myc is coupled to the MAX protein via its C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain (aa 413-434) and this heterodimer interacts with a number of other transcription-related proteins. In normal cells its expression and function are regulated by developmental or mitogenic signals. c-Myc mRNA has a very short half-life and in the absence of positive regulatory signals there is a significant reduction in its transcription leading to low c-Myc protein levels that does not have any proliferative drive. However, in tumor cells its levels are increased either via mutations or induction via upstream oncogenic pathways. Serine 347/348 are shown to be phosphorylated by casein kinase II. S347A/S348A reported to be more potent oncogenic mutants compared with wild-type c-Myc. Mutations leading to S347A/S348A are known to enhance transformation of cells compared to wild types. Mutations in c-Myc gene have been linked to B-cell chronic lymphocytic leukemia and Burkitt lymphoma. Over-expression of c-Myc has been reported in about 40% tumors. (Ref.: Wasylishen, AR., et al. (2013). Cancer Res. 73(21); 6504-6515; Miller, DM et al., (2012). Clin. Cancer Res. 18(20); 5546 5553; Lutterbach, B., and Hann, SR (1997). Oncogene. 14(8); 967-975).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho-c-Myc (Ser347), Cat. No. ABC1461, is a rabbit polyclonal antibody that detects c-Myc phosphorylated on serine 347 and is tested for use in Peptide Inhibition Assay and Western Blotting.
      Key Applications
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected phospho-c-Myc (Ser347) in HEK293T cells transfected with V5-WT-Myc and treated with MG132 (Courtesy of Dr. Linda Penn Lab @ Princess Margaret Cancer center, part of the University Health Network, Toronto).

      Peptide Inhibition Assay: Target band detection in lysate from HEK293T cells transfected with V5-WT-Myc and treated with MG132 (10 mM; 4 h) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the control peptide.


      Peptide Inhibition Assay: A 1:500 dilution from a representative lot was used with HEK293T cells transfected with V5-WT-Myc and treated with MG132 for peptide block analysis (Courtesy of Dr. Linda Penn Lab @ Princess Margaret Cancer Centre, part of the University Health Network, Toronto).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 347 from the C-terminal half of human c-Myc.
      EpitopeC-terminus
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects c-Myc phosphorylated on serine 347. It targets an epitope within 10 amino acids surrounding phosphoserine 347 from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Non-Human Primates based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYC
      • BHLHE39
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~58 kDa observed; 48.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T transfected with V5-WT-Myc and treated with MG132.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected c-Myc phosphorylated on serine 347 in lysate from HEK293T cells transfected with V5 tagged-wild-type c-Myc and treated with MG132 (10 µM, 4 h).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1461-25UL 04061842667574