Millipore Sigma Vibrant Logo

20-175 HDAC Assay Buffer, 5X

The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity & represses transcription when tethered to a promoter.

HDAC Assay Buffer, 5X MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Histone Deacetylase Assay (HDAC)
View Products on Sigmaaldrich.com
20-175
10 mL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      HDAC
      Description
      Catalogue Number20-175
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC Assay Buffer, 5X
      References
      Product Information
      Presentation5X HDAc Assay Buffer: 50mM Tris-HCl, pH 8.0, 750mM NaCl and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThe protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity & represses transcription when tethered to a promoter.
      Key Applications
      • Histone Deacetylase Assay (HDAC)
      Application NotesFor use in the Histone Deacetylase Assay (HDAC) Kit.
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • HDAC1
      • RPD3L1
      • GON-10
      • DKFZp686H12203
      • HD1
      • RPD3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
      SIZE: 1122 amino acids; 121992 Da
      SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity).
      PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. & Ubiquitinated. Polyubiquitination however does not lead to its degradation.
      SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size10 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      20-175 04053252032769

      Documentation

      HDAC Assay Buffer, 5X SDS

      Title

      Safety Data Sheet (SDS) 

      HDAC Assay Buffer, 5X Certificates of Analysis

      TitleLot Number
      HDAC Assay Buffer, 5X 2934466
      HDAC Assay Buffer, 5X - 3782151 3782151
      HDAC Assay Buffer, 5X - 3866435 3866435
      HDAC Assay Buffer, 5X - 4254272 4254272
      HDAC Assay Buffer, 5X - Any-lot Any-lot

      References

      Reference overviewPub Med ID
      A novel protein, Mpm1, of the mitochondria of the yeast Saccharomyces cerevisiae.
      H Inadome,Y Noda,H Adachi,K Yoda
      Bioscience, biotechnology, and biochemistry  65  2001

      Show Abstract       		11791739 11791739